Journal: The Journal of reproduction and development

Article Title: Follicular size is associated with the levels of transcripts and proteins of selected molecules responsible for the fertilization ability of oocytes of puberal gilts.

doi: 10.1262/jrd.20252

Figure Lengend Snippet: Fig. 2. Western-blot analysis. For western blot analysis, proteins were separated by SDS-PAGE and transferred to a nitrocellulose membrane that was then immunoblotted with goat polyclonal anti-pZP3 Ab (N-20; Fig. 2A) and goat polyclonal anti-integrin β2 Ab (C-20; Fig 2B), followed by incubation with donkey anti- goat HRP-conjugated Ab. To equalize protein loading, the membrane was reblotted with anti-actin HRP-conjugated Ab (Fig. 2C). Optical density (OD) was evaluated by using a Gel Logic 200 Imaging System (Kodak, Rochester, NY, USA). S, small follicles; M, medium follicles; L, large follicles.

Article Snippet: The membranes were also incubated with anti-actin HRP-conjugated Ab (clone I-19; Santa Cruz Biotechnology, Santa Cruz, CA, USA) to ensure equal protein loading of the lanes.

Techniques: Western Blot, SDS Page, Membrane, Incubation, Imaging

Journal: Clinical Ophthalmology (Auckland, N.Z.)

Article Title: Acute hemorrhagic conjunctivitis: anti-coxsackievirus A24 variant secretory immunoglobulin A in acute and convalescent tear

doi: 10.2147/OPTH.S85358

Figure Lengend Snippet: Immunoreactivity of tear and serum IgG (green) and IgA (red) from the AHC patient with CA24v-infected cells. Notes: Arrows show similar differential staining patterns for tear IgG and IgA in CA24v infected HRPE and AGMK cells. ( A ) Detection of immunoreactive human IgG and/or IgA in CA24v-infected HRPE cells treated with pooled acute tears (collected 1–4 days po). ( B ) Detection of immunoreactive human IgG and/or IgA in CA24v-infected AGMK cells treated with pooled acute tears. ( C ) Detection of immunoreactive human IgG and IgA in CA24v-infected HRPE cells treated with tears collected 4 years post-AHC. ( D ) Detection of immunoreactive human IgG and IgA in CA24v-infected AGMK cells treated with tears collected 5 years post-AHC. ( E ) Detection of antibody to secretory component (polymeric IgA) in CA24v-infected HRPE cells treated with pooled acute tears. ( F ) Detection of antibody to secretory component (polymeric IgA) in CA24v-infected HRPE cells treated with tears collected 5 years post-AHC. ( G ) Detection of CA24v immunoreactive human IgG and IgA in acute serum (collected 11 days po)-treated virus-infected HRPE cells. ( H ) Detection of CA24v immunoreactive human IgG and IgA in virus-infected HRPE cells treated with the patient’s convalescent serum (collected 5 years post-AHC). Bar =50 µm. Abbreviations: IgG, immunoglobulin G; IgA, immunoglobulin A; AHC, acute hemorrhagic conjunctivitis; CA24v, coxsackievirus A24 variant; HRPE, human retinal pigmented epithelial; po, post-onset; AGMK, African green monkey kidney.

Article Snippet: Human retinal pigmented epithelial (HRPE) cells (CRL-2502; ARPE-19) and African green monkey kidney (AGMK) cells were maintained as recommended by the provider; (American Type Culture Collection, Rockville, MD, USA). (It should be noted that cultured retinal pigmented epithelial cells express γFc receptor mRNA. )

Techniques: Infection, Staining, Virus, Variant Assay

Journal: Malaria Journal

Article Title: Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens

doi: 10.1186/s12936-022-04203-9

Figure Lengend Snippet: List of spiked samples in the blood and DBS panels used in this study

Article Snippet: Plasmodium falciparum W2 ( hrp2 + hrp3 + ) and Dd2 ( hrp2 – hrp3 + ) strains were obtained from Biodefense and Emerging Infections Research Resources Repository (BEI) Resources (Manassas, VA, USA) and the 3BD5 ( hrp2 – hrp3 – ) strain was obtained from the National Institute of Allergy and Infectious Diseases (Bethesda, Maryland, USA).

Techniques: Cell Culture, Expressing

Journal: Malaria Journal

Article Title: Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens

doi: 10.1186/s12936-022-04203-9

Figure Lengend Snippet: Inter-assay variability for malaria antigen signal intensity determined using Q-Plex and xMAP platforms

Article Snippet: Plasmodium falciparum W2 ( hrp2 + hrp3 + ) and Dd2 ( hrp2 – hrp3 + ) strains were obtained from Biodefense and Emerging Infections Research Resources Repository (BEI) Resources (Manassas, VA, USA) and the 3BD5 ( hrp2 – hrp3 – ) strain was obtained from the National Institute of Allergy and Infectious Diseases (Bethesda, Maryland, USA).

Techniques: Inter Assay

Journal: Malaria Journal

Article Title: Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens

doi: 10.1186/s12936-022-04203-9

Figure Lengend Snippet: Identification of P. falciparum mutants with hrp2/hrp3 deletions

Article Snippet: Plasmodium falciparum W2 ( hrp2 + hrp3 + ) and Dd2 ( hrp2 – hrp3 + ) strains were obtained from Biodefense and Emerging Infections Research Resources Repository (BEI) Resources (Manassas, VA, USA) and the 3BD5 ( hrp2 – hrp3 – ) strain was obtained from the National Institute of Allergy and Infectious Diseases (Bethesda, Maryland, USA).

Techniques:

Journal: Malaria Journal

Article Title: Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens

doi: 10.1186/s12936-022-04203-9

Figure Lengend Snippet: Comparative analyses of HRP2, PanLDH, Pf LDH, and Pv LDH in whole blood samples. A Linear regression scatter plots were generated from log transformed data for the WHO international standard P. falciparum and P. vivax antigens, P. falciparum culture, and clinical samples in whole blood. The equation of regression line and Pearson correlation r value were analysed using the paired sets of data for each analyte. B. Bland–Altman plots comparing the Q-Plex and xMAP assays for the aforementioned antigens. The mean difference between antigens’ concentrations (solid line) measured by the Q-Plex and xMAP and the 95% confidence interval of the difference (dotted lines) are shown. The samples that are far from a regression line are indicated as circles in plots and names of sample types in colors. Data from 3BD5 samples were excluded, as HRP2 was absent in these samples

Article Snippet: Plasmodium falciparum W2 ( hrp2 + hrp3 + ) and Dd2 ( hrp2 – hrp3 + ) strains were obtained from Biodefense and Emerging Infections Research Resources Repository (BEI) Resources (Manassas, VA, USA) and the 3BD5 ( hrp2 – hrp3 – ) strain was obtained from the National Institute of Allergy and Infectious Diseases (Bethesda, Maryland, USA).

Techniques: Generated, Transformation Assay

Journal: Malaria Journal

Article Title: Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens

doi: 10.1186/s12936-022-04203-9

Figure Lengend Snippet: Comparative analyses of HRP2, PanLDH, Pf LDH, and Pv LDH in DBS samples. A Linear regression scatter plots were generated from log transformed data for the WHO international standard P. falciparum and P. vivax antigens, P. falciparum culture, and clinical samples in DBS. The equation of regression line and Pearson correlation were analysed using the paired sets of data for each analyte. B Bland–Altman plots comparing the Q-Plex and xMAP assays for the aforementioned antigens. The mean difference between antigens’ concentrations (solid line) measured by the Q-Plex and xMAP and the 95% confidence interval of the difference (dotted lines) are shown. The samples in a cluster off a regression line are indicated as circles in plots and names of sample types in colors. Data from 3BD5 samples were excluded as HRP2 was absent in these samples

Article Snippet: Plasmodium falciparum W2 ( hrp2 + hrp3 + ) and Dd2 ( hrp2 – hrp3 + ) strains were obtained from Biodefense and Emerging Infections Research Resources Repository (BEI) Resources (Manassas, VA, USA) and the 3BD5 ( hrp2 – hrp3 – ) strain was obtained from the National Institute of Allergy and Infectious Diseases (Bethesda, Maryland, USA).

Techniques: Generated, Transformation Assay